Molecular Detection by RT-PCR<<Return to Laboratory
Molecular detection of hepatitis C virus by in house real-time (RT)-PCR targeting two regions of the viral genome (5’ UTR and 3’ UTR).
- Hepatitis C
Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected hepatitis C infection. Serology results must show markers to the hepatitis C virus.
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories. Where known, please include the viral load.
Real Time (RT) and conventional PCR with multiple Taqman primer sets.
19 calendar days.
- J. Garson. The polymerase chain reaction and hepatitis C virus diagnosis. FEMS Microbiology Reviews, 1994, 14; 229-240.
- Koppelman MH, Assal A, Chudy M, Torres P, de Villaescusa RG, Reesink HW, Lelie PN, Cuypers HT. Multicenter performance evaluation of a transcription-mediated amplification assay for screening of human immunodeficiency virus-1 RNA, hepatitis C virus RNA, and hepatitis B virus DNA in blood donations.Transfusion. 2005; 45:1258-66.