Genotyping by RT-PCR

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Requisition Forms

Reference Details

Description:

Genotyping of hepatitis C virus by conventional reverse-transcription-PCR followed by DNA sequencing.

Test Category:
Genotyping
Pathogen:
Hepatitis C virus (HCV)
Illnesses and Diseases:
  • Hepatitis C
Specimen:

Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected hepatitis C infection.  Serology results must show markers to the hepatitis C virus (anti-HCV (+)). If PCR was performed previously the result should be positive. If the viral load was previously determined, please include this information along with the request.

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Comments:

N/A

Methods and Interpretation of Results:

Conventional reverse transcription-PCR amplifying fragments of the Core, E1 and NS5B regions followed by sequencing.

Turnaround Time:

28 calendar days.

Contact:
Phone: (204) 789-6061; HCV (204) 789-5063
Fax: (204) 789-2082
References:
  1. Kuiken C, Simmonds P. Nomenclature and numbering of the hepatitis C virus. Methods Mol Biol. 2009;510:33-53.
  2. Simmonds P. Virology of hepatitis C virus. Clin Ther. 1996;18 Suppl B:9-36.
  3. Bowden DS, Berzsenyi MD. Chronic hepatitis C virus infection: genotyping and its clinical role. Future Microbiol. 2006;1:103-12.
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