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Antimicrobial Susceptibility Testing

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*Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Antimicrobial Susceptibility Testing (AST) on Group A Streptococcus.

Test Category:
Susceptibility Testing
Pathogen:
Streptococcus pyogenes
Illnesses and Diseases:
  • Bacteraemia
  • Invasive Group A Streptococcal Disease
  • Skin and soft tissue infections
  • Necrotizing fasciitis
  • Toxic shock syndrome
Specimen:

Pure culture isolated from a normally sterile site.

Specimens may be subject to rejection if they are not the appropriate sample type, have insufficient volume, or are not accompanied by relevant patient information.

Collection Method:

Isolate provided on culture medium.

Specimen Processing, Storage and Shipping:

Send as growth on culture medium (preferred) or on a swab in transport medium.  Chocolate or charcoal media are recommended.  Cultures can be also shipped frozen on dry ice. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Invasive disease due to Group A Streptococcus.

Accompanying Documentation:

Completed Isolate Submission Requisition for Streptococcus form including contact and lab name, address and telephone number.  Patient age or date of birth, sex, culture reference number, clinical source, date collected and test requested. 

Comments:

N/A

Methods and Interpretation of Results:

Prediction of antimicrobial susceptibility using whole genome sequencing (primary) (2)

*Antimicrobial susceptibility testing using Sensititre® plates (secondary) (1)

Turnaround Time:

30 calendar days**

Outbreak associated or urgent isolates will be granted priority status and completed as soon as possible.

**Turn around times may not be guaranteed when isolates are submitted in large batches.

Contact:
Phone #: (204) 789-6063; STI: (204) 784-5995
Fax: (204) 789-2140
References:
  1. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 31th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2021.
  2. Demczuk W, Martin I, Griffith A, Lefebvre B, McGeer A, Tyrrell GJ, Zhanel GG, Kus JV, Hoang L, Minion J, Van Caeseele P, Raafat Gad R, Haldane D, Zahariadis G, Mead K, Steven L, Strudwick L, Mulvey MR. Linear regression equations to predict β-lactam, macrolide, lincosamide and fluoroquinolone minimum inhibitory concentrations from molecular antimicrobial resistance determinants in Streptococcus pneumoniae. 2022;66(1):e0137021. doi: 10.1128/AAC.01370-21.