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Genomic subtyping

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*Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025).

Requisition Forms

Reference Details

Description:

Genomic subtyping of Vibrio species including Vibrio cholerae.

Test Category:
Molecular Differentiation
Pathogen:
Vibrio spp.
Laboratory:
Illnesses and Diseases:
  • Cholera
  • Virbriosis
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

Collection Method:

Isolate provided on appropriate culture medium for Vibrio species.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Gastroenteritis, septicemia, infection, other.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

N/A

Methods and Interpretation of Results:

Whole Genome MLST analysis (Vibrio cholerae only)*

Testing is performed, in whole or in part, using a “Research Use Only” testing kit which has not been fully validated/verified due to a lack of well-characterized panel.

Whole Genome SNV analysis (all Vibrio species)

Testing is performed, in whole or in part, using a “Research Use Only” testing kit which has not been fully validated/verified.

Data will be forwarded to the NML Surveillance, Outbreak Detection and Response section for interpretation according to PulseNet Canada guidelines.  Please contact us using the information below for further information on interpretation guidelines.

 

Turnaround Time:

21 calendar days for WGS performed in-house.  5 calendar days for analysis on samples sequenced provincially.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Contact:
Phone #: (204) 784-4827
Fax: (204) 789-5012
References: