Toxin detection

<<Return to Search Results

Requisition Forms

Reference Details

Description:

Toxin detection in Vibrio cholerae

Test Category:
Toxin Testing
Pathogen:
Vibrio cholerae
Illnesses and Diseases:
  • Cholera
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

Collection Method:

Isolate provided on appropriate culture medium for Vibrio species.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

Gastroenteritis, septicemia, infection, other

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

N/A

Methods and Interpretation of Results:

PCR-based identification of genes encoding toxins and virulence factors

ctx –Cholera toxin; causes severe diarrhea; indicative of toxigenic Vibrio spp.

vhlyA – Hemolysin A is a pore-forming exotoxin; widely thought to enhance virulence of V. cholerae (accessory toxin)

rtx  – repeat/accessory toxin; associated with toxin transportation

  1. Paton, A.W. and Paton, J. C. 1997. Detection and Characterization of Shiga Toxigenic Escherichia coli by using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E.coli hlyA, rfb0111, and rfb0157. J. of Clin. Micro. 36: 598 – 602.
Turnaround Time:

 7 calendar days. Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Contact:
Phone: (204) 789-6009
Fax: (204) 789-5012
References: