Identification

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*Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Identification of various, unidentified Enterbacteriaceae.

Test Category:
Phenotypic and Molecular Identification
Pathogen:
Enterobacteriaceae, Various Gram Negative
Illnesses and Diseases:
  • Gastroenteritis
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

Collection Method:

Isolate provided on appropriate culture medium with visible growth.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

Gastroenteritis, septicemia, infection, other.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

N/A

Methods and Interpretation of Results:

Phenotypic Identification (biotyping)*

16S rRNA gene sequencing

MALDI-TOF

Whole genome sequencing based methodologies may be applied.

  1. Biotyping: as per Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H., Manual of Clinical Microbiology. 2003. ASM Press. Washington, DC. USA
  2. Sequences compared to the 16s rDNA database in NCBI and clustered using BioNumerics
  3. MALDI-TOF results analysed using the most current Bruker Biotyper database.
Turnaround Time:

21 calendar days.

Contact:
Phone: (204) 789-6009
Fax: (204) 789-5012
References:
  1. Ewing, W.H. 1986. Edwards and Ewing's Identification of Enterobacteriaceae. 4th edition Elsevier Science Publishing Co., Inc., New York.
  2. Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H., Manual of Clinical Microbiology. 2003. ASM Press. Washington, DC. USA.
  3. Laboratory Methods For The Diagnosis Of Cholera Vibrio and Other Vibrios. 1991. Institut Pasteur 28, rue du Docteur-Roux 75724 PARIS CEDEX 15 - FRANCE.