Non-vanA/B VRE Confirmation

Confirmation of non-vanA/B vancomycin-resistant Enterococcus by PCR.

Pure culture.

Isolate provided on appropriate culture medium for growth.

Send cultures as growth on culture medium or in transport medium. Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

N/A

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

N/A

PCR is used to confirm presence or absence of VRE in isolates that are negative for vanA/B but phenotypically resistant. Results are compared to a known Standard. Vancomycin resistant genes tested include, vanD, vanN, vanL, vanE, and vanG.

6 calendar days.

1. Boyd, D.A., B.M. Willey, D. Fawcett, N. Gillani, and M.R. Mulvey. 2008. Molecular Characterization of Enterococcus faecalis N06-0364 with Low-Level Vancomycin Resistance Harboring a Novel D-Ala-D-Ser Gene Cluster, vanL. Antimicrobial Agents and Chemotherapy. 52:2667-72.