Molecular Detection of Vaccinia virus

Molecular detection of Vaccinia virus

• Vaccinia

Lesion fluid and/or crust, scab, skin material; swabs; CSF, other bodily fluids (minimum sample 0.5 mL) and fresh frozen tissues. Formalin-fixed or paraffin-embedded tissues.

Collect lesion or crust material, scabs, swabs, CSF in sterile 1.5-2.0 mL tube, dry swabs are recommended, transport media will be accepted if indicated on requisition.

Serum: collect in serum (red top) or serum separator tubes (SST), centrifugation of serum tubes is not needed and should be avoided.

CSF and other bodily fluids:  Collect in sterile 1.5-2.0 mL screw cap tubes.

Tissues: Fresh frozen tissues should be placed in plastic containers. Tissues that are formalin-fixed should also be sent in plastic containers and clearly identified as being in formalin. Paraffin-embedded tissues can be sent as entire blocks or four to six 10 uM sections in a plastic tube or vial.

Store samples in refrigerator or frozen until being shipped for testing.  Serum can be shipped refrigerated or frozen. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Presence of orthopoxvirus papules or lesions.

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Vaccinia PCR is validated. All other tests are non-routine test for investigational purposes.

For diagnosis of orthopoxvirus infection be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of orthopoxvirus DNA by PCR, (ii) isolation of orthopoxvirus.

14 calendar days.

Pathogen Safety Data Sheets: Infectious Substances – Vaccinia Virus