Isolation of Vaccinia virus<<Return to Laboratory
Isolation of Vaccinia virus
Lesion or crust fluid/material.
Collect lesion or crust material in sterile 1.5-2.0 mL tube, avoid excessive dilution of samples with viral transport media.
Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
Presence of orthopoxvirus papules or lesions.
Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.
All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.
For diagnosis of orthopoxvirus infection be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of orthopoxvirus DNA by PCR, (ii) isolation of orthopoxvirus.
14 calendar days.