Molecular Detection and Genotyping of Azithromycin Resistance

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Requisition Forms

Reference Details

Description:

Molecular detection and genotyping of azithromycin resistant Treponema pallidum using conventional PCR and DNA sequencing.

Test Category:
Molecular Detection and Genotyping
Pathogen:
Treponema pallidum subspecies pallidum
Illnesses and Diseases:
  • Syphilis
Specimen:

Swab, cerebrospinal fluid (CSF) or whole blood. Minimum of 0.5 mL of CSF. Minimum of 0.5 mL of whole blood.

Collection Method:

Use buccal or Dacron swabs to obtain specimens from ulcers.

Specimen Processing, Storage and Shipping:
Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice
Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected of syphilis infection.

Accompanying Documentation:

Completed Syphilis requisition form including sender lab name, address and telephone number. Patient identifier (specimen reference #), date of birth and sex. Date collected and test requested.

Comments:

Specimens that are positive by molecular detection using conventional PCR are genotyped to determine if they are resistant to azithromycin.

Methods and Interpretation of Results:

For research purposes only.

Turnaround Time:

20 calendar days.

Contact:
Phone: (204) 789-2130 or (204) 789-6017
Fax: (204) 789-2018
References:
Related Information: