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Molecular Detection and Genotyping of Azithromycin Resistance

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Reference Details


Molecular detection and genotyping of azithromycin resistant Treponema pallidum using conventional PCR and DNA sequencing.

Test Category:
Molecular Detection and Genotyping
Treponema pallidum subspecies pallidum
Illnesses and Diseases:
  • Syphilis

Swab, cerebrospinal fluid (CSF), whole blood or fresh tissue.  Minimum of 0.5 mL of CSF, minimum of 0.5 mL of whole blood, 1-5 g of tissue. For other specimen types, please call or email prior to shipping.

Specimens may be subject to rejection if they are not the appropriate sample type, have insufficient volume, or are not accompanied by relevant patient information.

Collection Method:

Use appropriate swab, such as Rayon or Dacron swabs to obtain specimens from ulcers.

Specimen Processing, Storage and Shipping:

Swabs can be shipped dry or in appropriate transport medium, such as viral transport medium.  Store samples refrigerated or frozen until shipped for testing.  Ship frozen samples on dry ice, and refrigerated samples on wet ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Suspected of syphilis infection.

Accompanying Documentation:

Completed Syphilis requisition form including sender lab name, address and telephone number. Patient identifier (specimen reference #), date of birth and sex. Date collected and test requested.


Specimens that are positive by molecular detection using conventional PCR are genotyped to determine if they are resistant to azithromycin.

Methods and Interpretation of Results:

For research purposes only.

Turnaround Time:

20 calendar days.

Phone #: (204) 789-2130
Fax: (204) 789-2018
Related Information: