Determination of emm type for Streptococcus pyogenes by PCR

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Requisition Forms

Reference Details

Description:

The determination of emm type for Streptococcus pyogenes by PCR.

Test Category:
Genotyping
Pathogen:
Streptococcus pyogenes
Illnesses and Diseases:
  • Bacteremia
  • Invasive Group A Streptococcal Disease
  • Skin and soft tissue infections
  • Toxic shock syndrome
  • Necrotizing fasciitis
Specimen:

Pure culture, presumptively identified as S. pyogenes, isolated from a normally sterile site (blood, CSF, pleural fluid, pericardial fluid, peritoneal fluid, deep tissue specimen or biopsy taken during surgery, bone or joint fluid) or any site (even if non-sterile) in a case of toxic shock syndrome or necrotizing fasciitis. Exclude middle ear and superficial wound aspirates.

Collection Method:

Isolate provided on culture medium.

Specimen Processing, Storage and Shipping:

Send as growth on culture medium (preferred) or on a swab in transport medium. Chocolate or charcoal media are recommended. Cultures can be also shipped frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

Invasive disease due to Streptococcus pyogenes.

Accompanying Documentation:

Completed Isolate Submission Requisition for Streptococcus form including sender lab name, address and telephone number.  Patient age or date of birth, sex, culture reference number, clinical source, date collected and test requested. 

Comments:

Non-invasive isolates of Streptococcus pyogenes that are related to outbreak investigations may be submitted.

Methods and Interpretation of Results:

Based on the following references and Standard Operating Procedures (SOPS).

Turnaround Time:

30 calendar days. Outbreak associated or urgent isolates will be granted priority status and completed as soon as possible. *Turn around times may not be guaranteed when isolates are submitted in large batches.

Contact:
Phone: (204) 789-6063; STI: (204) 784-5995
Fax: (204) 789-2140
References:
  1. Beall, Bernard, Gherardi, G., Lovgren, M., Facklam R.R., Forwick B.A. and Tyrrell, G.J. emm and sof gene sequence variation in relation to serological typing of opacity-factor-positive group A streptococci. Microbiology 2000;146:1195-1209.
  2. Centers for Disease Control and Prevention, Streptococcus Laboratory. Streptococcus Laboratory Protocols. Available at http://www.cdc.gov./ncidod/biotech/strep/protocols.htm