Serotype determination of Streptococcus pneumoniae by Quellung reaction

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Serotype determination of Streptococcus pneumoniae by Quellung reaction.

Test Category:
Serotyping
Pathogen:
Streptococcus pneumoniae
Illnesses and Diseases:
  • Bacteremia
  • Pneumococcal meningitis
Specimen:

Pure culture, presumptively identified as S. pneumoniae, isolated from a normally sterile site (excluding the middle ear). Isolate provided on culture medium

Collection Method:

Isolate provided on culture medium.

Specimen Processing, Storage and Shipping:

Send as growth on culture medium (preferred) or on a swab in transport medium. Chocolate or charcoal media are recommended. Cultures can be also shipped frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

Invasive disease due to Streptococcus pneumoniae.

Accompanying Documentation:

Completed Isolate Submission Requisition for Streptococcus form including contact and lab name, address and telephone number.  Patient age or date of birth, sex, culture reference number, clinical source, date collected and test requested. 

Comments:

Non-invasive isolates of Streptococcus pneumoniae that are related to outbreak investigations may be submitted.

Methods and Interpretation of Results:

Based on the following references and Standard Operating Procedures (SOPS).

Turnaround Time:

30 calendar days. Outbreak associated or urgent isolates will be granted priority status and completed as soon as possible. *Turn around times may not be guaranteed when isolates are submitted in large batches.

Contact:
Phone: (204) 789-6063; STI: (204) 784-5995
Fax: (204) 789-2140
References:
  1. Austrian, R., Boettger, M. Dole, L. Fairly, and M. Fried. 1985. Streptococcus pneumoniae type 16A, a hitherto undescribed pneumococcal type. J. Clin. Microbiol. 22:127-128.
  2. Henrichsen, J. 1995. Six newly recognized types of Streptococcus pneumoniae. J. Clin. Microbiol. 33:2759-2762.
  3. Lund, E. Henrichsen J. Laboratory diagnosis, serology and epidemiology of Streptococcus pneumoniae. In: Bergan T. Norris JR, eds. Methods in Microbiology. London: Academic Press, 1978:241-262.
  4. Oftadeh,S., Satzke,C.,Gilbert,G. 2010. Identification of newly described Streptococcus pneumoniae serotype 6D using Quellung reaction and PCR. J. Clin. Microiol. 48:3378-3379.
  5. World Health Organization. 2003. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health concern in the developing world.  Chapter 5.  Streptococcus pneumoniae Confirmatory Identification and Antimicrobial Susceptibility Testing. p45-62
  6. Available at: ttp://www.who.int/csr/resources/publications/drugresist/WHO_CDS_CSR_RMD_2003_6/en/

  7. World Health Organization. 2003. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health concern in the developing world.  Appendix 6.  Serotyping and Quellung Typing of Streptococcus pneumoniae. p 255-258.  Available at: http://www.who.int/csr/resources/publications/drugresist/WHO_CDS_CSR_RMD_2003_6/en/
  8. Lo SW, Gladstone RA, van Tonder AJ, et al. Global Distribution of Invasive Serotype 35D Streptococcus pneumoniae Isolates following Introduction of 13-Valent Pneumococcal Conjugate Vaccine. J Clin Microbiol. 2018;56(7):e00228-18. Published 2018 Jun 25. doi:10.1128/JCM.00228-18
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