Molecular Detection of Sin Nombre virus

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Requisition Forms

Reference Details

Description:

Amplification of Sin Nombre virus specific genome.

Test Category:
Molecular Detection
Pathogen:
Sin Nombre virus
Illnesses and Diseases:
  • Hantavirus cardiopulmonary syndrome (HPS)
Specimen:

Whole blood or lung tissue are preferred samples, sera and BAL also acceptable.

 

Collection Method:

Collect in serum separator tubes (SST) and whole blood in EDTA tubes, can submit as aliquots in sterile 1.5-2.0 mL tube. For PCR from tissue samples, please indicate if the sample is formalin fixed.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

History of contact to rodents or their droppings.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Methods and Interpretation of Results:

For diagnosis of Hantavirus pulmonary syndrome (HPS) or hemorrhagic fever with renal syndrome (HFRS) to be confirmed , one or more of the following diagnostic markers must be positive: (i) presence of hantavirus-specific IgM or rising titers of IgG, (ii) presence of hantavirus RNA by PCR.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6019 or (204) 789-7032
Fax: (204) 789-2140
References: