Toxin detection<<Return to Laboratory
*Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)
Toxin detection in Shigella spp.
Isolate from urine, stool, blood, environmental, food, other.
Isolate provided on appropriate culture medium for Shigella.
Send as growth on appropriate culture medium or in appropriate transport medium. Culture vessel should be leak-proof or sealed appropriately.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Completed Division of Enteric Diseases requisition form.
PCR amplification of:
- toxin genes (stx1, stx2)* (1)
- virulence genes (eaeA, hlyA, ipaH & ial)* (1,2)
- Paton, A.W. and Paton, J. C. 1997. Detection and Characterization of Shiga Toxigenic Escherichia coli by using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E.coli hlyA, rfb0111, and rfb0157. J. of Clin. Micro. 36: 598 – 602.
- Sethabutr, O. et al. 1993. Detection of Shigellae and Enteroinvasive Escherichia coli by Amplification of the Invasion Plasmid Antigen H DNA Sequence in Patients with Dysentery. J. of Infect. Dis. Jan 167; 458-461.
7 calendar days. Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.