Detection of antibodies against Seoul virus by ELISA
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Detection of antibodies against Seoul virus by Enzyme Linked Immunosorbent Assay (ELISA).
- Hemorrhagic fever with renal syndrome (HFRS)
Serum or plasma - minimum sample 0.5 mL.
Collect in serum (red top) or serum separator tubes (SST), centrifugation of serum tubes is not needed and should be avoided.
Store samples in refrigerator or frozen until being shipped for testing. Serum can be shipped refrigerated or frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Symptomatic and history of contact to rodents or their droppings.
Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.
Serology is considered the gold standard for identifying a Hantavirus infection, all PCR requests must be run in parallel by serology with the appropriate sample type.
All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.
For diagnosis of Hantavirus pulmonary syndrome (HPS) or hemorrhagic fever with renal syndrome (HFRS) to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of hantavirus-specific IgM or rising titers of IgG, (ii) presence of hantavirus RNA by PCR.
14 calendar days.