Serotyping
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Requisition Forms
Reference Details
Serotyping of Salmonella spp.
- Gastroenteritis
- Salmonellosis
Isolate from urine, stool, blood, environmental, food, other.
Isolate provided on appropriate culture medium for Salmonella.
Send as growth on appropriate culture medium or in appropriate transport medium. Culture vessel should be leak-proof or sealed appropriately.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Envoyer sur un milieu de culture ensemencé ou sur un milieu de transport approprié. Le récipient de culture doit être étanche ou adéquatement scellé.
Completed Enteric Diseases Program requisition form.
N/A
Salmonella in-silico typing resource (SISTR) (primary)
Agglutination assay (secondary/confirmatory)*
WGS data will be analyzed using the SISTR platform (SISTR is available on a public Galaxy instance at https://lfz.corefacility.ca/sistr-app/). Any serotype that cannot be confirmed using SISTR will be referred for phenotypic analysis using the “Antigenic Formulas of the Salmonella Serovars. 2007. WHO Collaborating Centre for Reference and Research on Salmonella. Michel Y. Popoff. Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex15, France“.
21 calendar days. Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.