Serotyping

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Serotyping of Salmonella spp.

Test Category:
Serotyping
Pathogen:
Salmonella spp.
Illnesses and Diseases:
  • Gastroenteritis
  • Salmonellosis
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

Collection Method:

Isolate provided on appropriate culture medium for Salmonella.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Envoyer sur un milieu de culture ensemencé ou sur un milieu de transport approprié.  Le récipient de culture doit être étanche ou adéquatement scellé.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

N/A

Methods and Interpretation of Results:

Salmonella in-silico typing resource (SISTR) (primary)

Agglutination assay (secondary/confirmatory)

WGS data will be analyzed using the SISTR platform (https://lfz.corefacility.ca/sistr-app/).  Any serotype that cannot be confirmed using SISTR will be referred for phenotypic analysis using the “Antigenic Formulas Of The Salmonella Serovars. 2007. WHO Collaborating Centre for Reference and Research on Salmonella. Michel Y. Popoff. Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex15, France“. 

Turnaround Time:

21 calendar days.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Contact:
Phone: (204) 789-6009
Fax: (204) 789-5012
References: