Identification

Identification of suspected Salmonella species.

Isolate from urine, stool, blood, environmental, food, other.

Isolate provided on appropriate culture medium for Salmonella.

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Gastroenteritis, septicemia, infection, other.

Completed Division of Enteric Diseases requisition form.

N/A

Phenotypic Identification (biotyping)(1)

Salmonella in-silico typing resource (SISTR)(2)                                           

MALDI-TOF (3)

Whole genome sequencing based methodologies may be applied

1. Biotyping: as per Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H., Manual of Clinical Microbiology. 2003. ASM Press. Washington, DC. USA
2. Yoshida CE, Kruczkiewicz P, Laing CR, Lingohr EJ, Gannon VPJ, Nash JHE, et al. (2016) The Salmonella In Silico Typing Resource (SISTR): An Open Web-Accessible Tool for Rapidly Typing and Subtyping Draft Salmonella Genome Assemblies. PLoS ONE 11(1): e0147101. https://doi.org/10.1371/journal.pone.0147101
3. MALDI-TOF results analysed using the most current Bruker Biotyper database.

*Note: All isolates identified as Salmonella will be referred for whole genome sequencing and in silico serotyping. Traditional serotyping is also available but must be explicitly requested by submitting lab**

21 calendar days.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.