Detection of IgG Antibodies Directed Towards Ross River Virus by ELISA

Serological detection of IgG antibodies directed towards Ross River virus (RRV) by PanBio IgG ELISA.

• Ross River fever

Serum. Minimum volume of 250 µl required.

2 mL screw cap tubes.

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Ross River virus infection and relevant travel history.

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, clinical and travel history, and dates of travel of the patient.

All RRV diagnostic samples are tested by both IgM and IgG ELISA to increase the clinical sensitivity.

This test is considered investigational as it has not been extensively validated or verified.

Testing is performed, in whole or in part, using a “Research Use Only” testing kit which has not been fully validated/verified due to a lack of well-characterized panel.

The detection of IgG antibodies in a single serum sample is indicative of past or present exposure to this virus. A 4 fold rise or greater in neutralizing antibody titre, or seroconversion in paired sera, is required to document a "confirmed case" of infection.

IgM can persist in serum for up to a year or more after arbovirus exposure.  Thus, detection of IgM by itself is not sufficient for confirmation of acute infection, but is consistent with an exposure at an undetermined time.

Isolation of an arbovirus or detection of nucleic acid by real-time RT-PCR in a clinical specimen provides clear evidence of infection associated with the current clinical illness.

14 Calendar days.

1. Inverness Medical Innovations Australia Pty Ltd. 2008. Ross River Virus IgG ELISA kit instructions.

Pathogen Safety Data Sheets: Infectious Substances – Ross river virus