Molecular Detection by Real-Time PCR and Speciation by Conventional PCR<<Return to Laboratory
Molecular detection by real-time PCR and speciation of Rickettsial species.
- Rocky Mountain spotted fever (RMSF)
- African tick bite fever
- Mediterranean spotted fever
- Rickettsial pox
- Spotted fever rickettsiosis
Whole blood – minimum 1.0 mL. Tissue – biopsy of eschar.
Whole blood – EDTA tube. Tissue or Swab – submit in a sterile collection tube.
Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped. Ship whole blood at 4°C and ship tissues frozen.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
Suspected rickettsial infection.
Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
Positive samples are speciated using conventional PCR and sequencing.
Real-time PCR is performed using assays specific for the 17 kDa antigen gene and citrate synthase gene of the Rickettsia genus. If a sample is positive by real-time PCR, conventional PCR and sequencing of the outer membrane protein A gene will be performed to determine the exact spotted fever group species. For the specific detection of R. typhi, an assay specific for the outer membrane protein B gene is performed.
15 calendar days.
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- Stenos, J, SR Graves, NB Unsworth. A highly sensitive and specific real-time PCR assay for the detection of spotted fever and typhus group rickettsia. Am J Trop Med Hyg 2005; 73(6):1083-1085.
- Henry KM, J Jiang, PJ Rozmajzl, AF Azad, KR Macaluso, AL Richards. Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Mol Cell Probes 2007; 21(1):17-23.
- Wikswo ME, R Hu, GA Dasch, L Krueger, A Arugay, K Jones, B Hess, S Bennett, V Kramer, ME Eremeeva. Detection and identification of spotted fever group rickettsiae in Dermacentor species from Southern California. J Med Entomol; 45(3): 509- 516.
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