Molecular Detection by Real-Time PCR and Speciation by Conventional PCR<<Return to Search Results
Molecular detection by real-time PCR and speciation of Rickettsial species.
- Rocky Mountain spotted fever (RMSF)
- African tick bite fever
- Mediterranean spotted fever
- Rickettsial pox
- Spotted fever rickettsiosis
Whole blood – minimum 1.0 mL. Tissue – biopsy of eschar. Swab – cutaneous swab of skin lesion after removal of eschar scab.
Whole blood – EDTA tube. Tissue or Swab – submit in a sterile collection tube.
Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped. Ship whole blood at 4°C and ship tissues frozen.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected rickettsial infection.
Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
Positive samples are speciated using conventional PCR and sequencing.
Real-time PCR is performed using assays specific for the 17 kDa antigen gene and citrate synthase gene of the Rickettsia genus. If a sample is positive by real-time PCR, conventional PCR and sequencing of the outer membrane protein A gene will be performed to determine the exact spotted fever group species. For the specific detection of R. typhi, an assay specific for the outer membrane protein B gene is performed.
15 calendar days.
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