Molecular detection of Ricinus communis by real-time PCR

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Molecular detection of Ricinus communis by real-time PCR

Test Category:
Molecular Detection
Pathogen:
Ricinus communis
Illnesses and Diseases:
  • Ricin poisoning
Specimen:

Whole blood – minimum 0.5 mL, powder, liquid, food sample

Collection Method:

Whole blood – EDTA tube; powder/liquid – submit in a sterile 2 mL o-ring microcentrifuge tube; food – submit in an appropriately sized container with an o-ring

Specimen Processing, Storage and Shipping:

Store blood samples refrigerated until shipped for testing.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected ricin infection

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.

Comments:

N/A

Methods and Interpretation of Results:

Real-time PCR is performed using in-house differentiating molecular assays with three targets specific for ricin DNA.

Confirmatory tests of a sample positive for ricin by real-time PCR result include Time Resolved Fluorescence (TRF) assay and/or Ricin Antigen Capture ELISA.

Turnaround Time:

4 calendar days for a preliminary result; 14 calendar days for a final result; 28 days for any non-standard specimen type or specialized testing requests.

Contact:
Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
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