Rabies Serum Neutralization Assay

Serology for detection of neutralizing antibodies to rabies virus.

• Rabies

Serum sample, cerebrospinal fluid (CSF). Minimum volume required – 2 mL.

Submit in a sterile microcentrifuge tube.

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Individuals who have received pre- or post-exposure vaccination for rabies virus. The Rabies, Rickettsia and Related Zoonotic Diseases laboratory must be notified prior to the submission of any samples for clinical investigation.

Completed Rabies Serum Neutralization Assay requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, occupational category, vaccination history, administration of RIG, animal exposure, travel history. Type of specimen and date collected.

Samples for post exposure vaccination will not be tested unless all requested information is supplied. The Rabies, Rickettsia and Related Zoonotic Diseases laboratory must be notified prior to the submission of any samples for clinical investigation.

Neutralizing antibody titres are reported in International Units/ml (IU/ml). Booster vaccination is recommended if antibody titre falls below 0.5 IU/ml. Individuals with antibody levels between 0.5 IU/ml and 1.0 IU/ml should be carefully assessed for their risk of exposure; if a booster dose of vaccine is not administered, a new serum sample should be drawn and tested within 3-6 months to ensure that the individual's antibody level does not fall below 0.5 IU/ml.

30 calendar days.

1. Statement on travellers and rabies vaccine. Canada Communicable Disease Report. 2002; 28: 1-12.

Pathogen Safety Data Sheets: Infectious Substances – Rabies virus

National case definition: Rabies

Rabies

Rabies and Travel