Molecular Detection by Real-Time PCR<<Return to Laboratory
Molecular detection of Orientia tsutsugamushi by real-time PCR.
- Scrub typhus
Whole blood – EDTA tube. Tissue or Swab – submit in a sterile collection tube.
Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped. Ship whole blood at 4°C and ship tissues frozen
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
Suspected Scrub Typhus infection; history of travel to an endemic region.
Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier(referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
Real-time PCR is performed using an assay specific forthe 47 kDa outer membrane protein gene of O. tsutsugamushi.
15 calendar days.
- Jiang J, TC Chan, JJ Temenak, GA Dasch, WM Ching, AL Richards. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. Am J Trop Med Hyg 2004; 70(4): 351-356.