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Molecular Detection by Real-Time PCR

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Molecular detection of Orientia tsutsugamushi by real-time PCR.

Test Category:
Molecular Detection
Orientia tsutsugamushi
Illnesses and Diseases:
  • Scrub typhus
Whole blood – minimum 1.0 mL. Tissue – biopsy of eschar.  Swab – cutaneous swab of skin lesion after removal of eschar scab.
Collection Method:

Whole blood – EDTA tube. Tissue or Swab – submit in a sterile collection tube.

Specimen Processing, Storage and Shipping:

Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped. Ship whole blood at 4°C and ship tissues frozen

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Suspected Scrub Typhus infection; history of travel to an endemic region.

Accompanying Documentation:

Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier(referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.

Methods and Interpretation of Results:

Real-time PCR is performed using an assay specific forthe 47 kDa outer membrane protein gene of O. tsutsugamushi.

Turnaround Time:

15 calendar days.

Phone #: (204) 789-7037
Fax: (204) 789-2018
  1. Jiang J, TC Chan, JJ Temenak, GA Dasch, WM Ching, AL Richards. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. Am J Trop Med Hyg 2004; 70(4): 351-356.
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