Molecular Detection of Orf virus

Amplification of Orf virus specific genome.

Lesion fluid and/or crust, scab, skin material; swabs; CSF, other bodily fluids (minimum sample 0.5 mL) and fresh frozen tissues  Formalin-fixed or paraffin-embedded tissues.

Prélever le tissu des lésions ou une partie des croûtes ou des gales et placer les écouvillons dans des tubes stériles de 1,5 à 2,0 ml. Il est recommandé d’utiliser des écouvillons secs, mais les échantillons conservés dans un milieu de transport sont acceptés si le milieu est précisé sur la demande.

LCR et autres liquides organiques : Prélever les échantillons dans des tubes stériles à bouchon à vis de 1,5 à 2,0 ml.

Tissus : Les tissus frais congelés doivent être placés dans des récipients en plastique. Les tissus fixés au formol doivent également être envoyés dans des conteneurs en plastique et être clairement identifiés comme étant fixés au formol. Les tissus inclus en paraffine peuvent être envoyés en blocs entiers ou en quatre à six sections de 10 µm dans un tube ou un flacon en plastique.

Store samples in refrigerator or frozen until being shipped for testing.  Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Formalin-fixed samples can be sent at room temperature.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Presence of parapoxvirus papules or lesions.

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk (contact to animals) and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

PCR is a non-routine test for investigational purposes.

Testing is performed, in whole or in part, using a lab-developed test which has not been fully validated/verified.

For diagnosis of parapoxvirus infection be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of parapoxvirus DNA by PCR, and (ii) isolation of parapoxvirus.

14 calendar days.