Molecular Detection of Orf virus

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Requisition Forms

Reference Details

Description:

Amplification of Orf virus specific genome.

Test Category:
Molecular Detection
Pathogen:
Orf virus
Illnesses and Diseases:
  • Orf infection
Specimen:

Lesion fluid and/or crust, scab, skin material; swabs; fresh frozen tissues – Minimum sample 0.5 mL.  Formalin-fixed or paraffin-embedded tissues.

Collection Method:

Collect lesion or crust material, scabs, swabs in sterile 1.5-2.0 mL tube, avoid dilution of the sample, transport media is not needed.

Tissues: Fresh frozen tissues should be placed in plastic containers. Tissues that are formalin-fixed should also be sent in plastic containers and clearly identified as being in formalin. Paraffin-embedded tissues can be sent as entire blocks or four to six 10 uM sections in a plastic tube or vial.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing.  Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Formalin-fixed samples can be sent at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Presence of parapoxvirus papules or lesions.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk (contact to animals) and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Methods and Interpretation of Results:

For diagnosis of parapoxvirus infection be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of parapoxvirus DNA by PCR, and (ii) isolation of parapoxvirus.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6019 or (204) 789-7032
Fax: (204) 789-2140
References:
Fact Sheets:
Guidelines: