Molecular Detection of Omsk Hemorrhagic Fever virus by PCR

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Description:

Detection of Omsk HF virus by PCR amplification of Omsk HF virus specific genome.

Test Category:
Molecular Detection
Pathogen:
Omsk hemorrhagic fever virus
Illnesses and Diseases:
  • Omsk hemorrhagic fever
Specimen:

Whole blood, serum, fresh frozen tissues – Minimum sample 0.5 mL.  Formalin-fixed or paraffin-embedded tissues.

 

Collection Method:

Whole blood: collect in EDTA, heparin or citrate tubes, can submit as aliquots in sterile 1.5-2.0 mL screw cap tubes.

Serum: collect in serum (red top) or serum separator tubes (SST), centrifugation of serum tubes is not needed and should be avoided.

Tissues: Fresh frozen tissues should be placed in plastic containers. Tissues that are formalin-fixed should also be sent in plastic containers and clearly identified as being in formalin. Paraffin-embedded tissues can be sent as entire blocks or four to six 10 uM sections in a plastic tube or vial.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing.  Serum can be shipped refrigerated or frozen. Avoid freezing whole blood samples. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Formalin-fixed samples can be sent at room temperature.

Transportation of Dangerous Goods:

Store samples in refrigerator or frozen until being shipped for testing. Ship frozen specimens on dry ice and refrigerated samples on wet ice. Shipping of samples suspected of containing Risk Group 4 pathogens shall be done by a TDG certified individual in accordance with TDG regulations. If applicable, initiate an Emergency Response Assistance Plan (ERAP) notification 24 hours prior to shipping. To initiate an ERAP notification, please click the link below to access the transport flow chart for Risk Group 4 pathogens or call 1-866-262-8433 (available 24/7) for assistance.

Transport Flowchart for Risk Group 4 Pathogen(s)
Patient Criteria:

Symptomatic and travel to Omsk HF virus endemic areas or contact to travellers from these areas.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection. Contact Special Pathogens prior to submission of sample to ensure appropriate shipping documentation is generated.

Methods and Interpretation of Results:

For diagnosis of Omsk HF infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of Omsk HF RNA by PCR, and (ii) isolation of Omsk HF virus.

Turnaround Time:

2 calendar days.

Contact:
Phone: (204) 789-6019 or (204) 789-7032
Fax: (204) 789-2140
References:
Related Information: