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Sequence Typing of Porin Genes

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Requisition Forms

Reference Details

Description:

Molecular typing of Neisseria meningitidis by DNA sequencing of target porin genes porA.

Test Category:
Molecular Typing
Pathogen:
Neisseria meningitidis
Illnesses and Diseases:
  • Meningitis
Specimen:

Pure culture.

Specimens may be subject to rejection if they are not the appropriate sample type or are not accompanied by relevant patient information.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send as growth on culture medium (preferred) or on a swab in transport medium. Cultures can be also shipped frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Suspected of invasive disease caused by Neisseria meningitidis.

Accompanying Documentation:

Completed Vaccine Preventable Bacterial Diseases requisition form including sender lab name, address, fax number and telephone number.  Patient identifier (specimen reference #), patient initials, date of birth and sex. Type of specimen, source of isolation, date collected, and test requested.  Vaccine history (if known).

Comments:

N/A

Methods and Interpretation of Results:

For research purposes only.

Turnaround Time:

20 calendar days (unless specified as urgent).

Contact:
Phone #: (204) 789-2130
Fax: (204) 789-2018
References:
  1. Sacchi CT, Lemos AP, Brandt ME, Whitney AM, Melles CE, Solari CA, Frasch CE, Mayer LW. 1998. Proposed standardization of Neisseria meningitidis PorA variable-region typing nomenclature. Clin Diagn Lab Immunol. Nov;5(6):845-55.
  2. Sacchi CT, Lemos AP, Whitney AM, Solari CA, Brandt ME, Melles CE, Frasch CE, Mayer LW.  1998.  Correlation between serological and sequencing analyses of the PorB outer membrane protein in the Neisseria meningitidis serotyping system.  Clin Diagn Lab Immunol. May;5(3):348-54.