Enzyme Linked Immunosorbant Assay (ELISA) - IgG

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Requisition Forms

Reference Details

Description:

Detection of IgG antibodies to the mumps virus by enzyme linked immunosorbant assay (ELISA), including measurement of IgG titres, if requested.

Test Category:
Serology
Pathogen:
Mumps virus
Illnesses and Diseases:
  • Mumps
Specimen:

Serum or plasma (100 µL minimum) collected as soon as possible after symptom onset.  For seroconversion, or determination of a change in IgG titre, submit acute and convalescent sera.  Acute serum should be collected immediately upon disease onset (within the first 7 days)   and convalescent serum should be collected 10 to 30 days later.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Follow standard laboratory techniques to prepare serum or plasma. Store at 4°C and ship at room temperature or on wet ice for arrival at the NML within 3 days of collection. Otherwise, freeze (-20°C or below) and ship frozen on dry ice.

 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:
  1. Patient with mumps IgM positive serology result, recent mumps vaccination, or recent contact with laboratory confirmed case of mumps.
  2. For seroconversion: suspected mumps case without an RT-PCR result and a negative or unreliable IgM result.
Accompanying Documentation:

Completed Measles, Mumps, and Rubella requisition form. Include date of disease onset, number of doses of mumps vaccine received, date of last mumps vaccination, travel history.

Comments:

Special request only. Kits are ordered on an as needed basis so please contact the lab in advance of sending samples for testing.

Methods and Interpretation of Results:

Testing is done using a commercial ELISA assay. A four-fold rise in IgG titre, or seroconversion (i.e. a negative and a positive result in acute and convalescent sera respectively), is indicative of infection.

Turnaround Time:

21 calendar days. Turnaround time can be as short as 3 calendar days, with proper notification.

Contact:
Phone: (204) 789-6024 or (204) 789-7055
Fax: (204) 318-2222
References:
  1. Public Health Agency of Canada. Laboratory guidelines for the diagnosis of mumps. CCDR. 2010; 36S1: 37-41.
  2. Tipples G, J Hiebert. Detection of measles, mumps and rubella viruses. Methods Mol Biol. 2011; 665: 183-193.
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