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Enzyme Linked Immunosorbant Assay (ELISA) - IgG

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Requisition Forms

Reference Details

Description:

Detection of IgG antibodies to the mumps virus by enzyme linked immunosorbant assay (ELISA), including measurement of IgG titres, if requested.

Test Category:
Serology
Pathogen:
Mumps virus
Illnesses and Diseases:
  • Mumps
Specimen:

Serum or plasma (100 µL minimum) collected as soon as possible after symptom onset.  For seroconversion, or determination of a change in IgG titre, submit acute and convalescent sera.  Acute serum should be collected immediately upon disease onset (within the first 7 days)   and convalescent serum should be collected 10 to 30 days later.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Follow standard laboratory techniques to prepare serum or plasma. Store at 4°C and ship at room temperature or on wet ice for arrival at the NML within 3 days of collection. Otherwise, freeze (-20°C or below) and ship frozen on dry ice.

 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

 

Patient Criteria:
  1. Patient with mumps IgM positive serology result, recent mumps vaccination, or recent contact with laboratory confirmed case of mumps.
  2. For seroconversion: suspected mumps case without an RT-PCR result and a negative or unreliable IgM result.
Accompanying Documentation:

Completed Measles, Mumps, and Rubella requisition form. Include date of disease onset, number of doses of mumps vaccine received, date of last mumps vaccination, travel history.

Comments:

Special request only. Kits are ordered on an as needed basis so please contact the lab in advance of sending samples for testing.

Methods and Interpretation of Results:

Testing is performed, in whole or in part, using an IVD testing kit which has not been fully validated/verified.

Testing is done using a commercial ELISA assay. A four-fold rise in IgG titre, or seroconversion (i.e. a negative and a positive result in acute and convalescent sera respectively), is indicative of infection.

Turnaround Time:

21 calendar days. Turnaround time can be as short as 3 calendar days, with proper notification.

Contact:
Phone #: (204) 789-6024 or (204) 789-7055
Fax: (204) 318-2222
References:
  1. Public Health Agency of Canada. Laboratory guidelines for the diagnosis of mumps. CCDR. 2010; 36S1: 37-41.
  2. Tipples G, J Hiebert. Detection of measles, mumps and rubella viruses. Methods Mol Biol. 2011; 665: 183-193.
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