Isolation of Monkeypox virus
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Reference Details
Isolation of monkeypox virus.
- Monkeypox
Lesion fluid, crust material.
Collect samples in sterile 1.5-5.0 mL tubes. Avoid excessive dilution of the sample in viral transport media.
Store samples in refrigerator or frozen until being shipped for testing. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Avoid freezing whole blood samples.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/
Symptomatic and travel to monkeypox virus endemic areas or contact to travellers or animals from these areas.
Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.
All patient history must be included; sample without adequate patient history to justify testing are subject to rejection. Contact Special Pathogens prior to submission of sample to ensure appropriate shipping documentation is generated.
For diagnosis of monkeypox virus infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of monkeypox virus DNA by PCR, and (ii) isolation of monkeypox virus.
2 calendar days.