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Molecular Detection of Molluscum contagiosum virus

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Requisition Forms

Reference Details

Description:

Amplification of Molluscum contagiosum genome.

Test Category:
Molecular Detection
Pathogen:
Molluscum contagiosum virus (MCV)
Illnesses and Diseases:
  • Molluscum contagiosum
Specimen:

Scab, skin material, swabs, CSF, other bodily fluids (minimum sample 0.5 mL) and fresh frozen tissues; formalin-fixed or paraffin-embedded tissues.

Collection Method:

Collect lesion or crust material, scabs, swabs in sterile 1.5-2.0 mL tube, dry swab recommended but transport media will be accepted if indicated on requisition.

CSF and other bodily fluids:  Collect in sterile 1.5-2.0 mL screw cap tubes

Tissues: Fresh frozen tissues should be placed in plastic containers. Tissues that are formalin-fixed should also be sent in plastic containers and clearly identified as being in formalin. Paraffin-embedded tissues can be sent as entire blocks or four to six 10 uM sections in a plastic tube or vial.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Formalin-fixed samples can be sent at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Presence of lesions consistent with Molluscum contagiosum infection.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases and travel history. Indicate type of specimen collected and all specimen information. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; samples without adequate patient history to justify testing are subject to rejection.

PCR test is validated.

Methods and Interpretation of Results:

For laboratory diagnosis of infection, Molluscum contagiosum DNA must be detected by PCR.

Turnaround Time:

14 calendar days.

Contact:
Phone #: (204) 789-6019 or (204) 789-7032*
Fax: (204) 789-2140
References:
Fact Sheets:
Guidelines:
Related Information: