Pulsed Field Gel Electrophoresis (PFGE)

<<Return to Laboratory

Requisition Forms

Reference Details

Description:

Pulsed-field gel electrophoresis of methicillin-resistant Staphylococcus aureus.

Test Category:
Genetic Fingerprinting
Pathogen:
Methicillin-resistant Staphylococcus aureus (MRSA)
Illnesses and Diseases:
  • Necrotizing pneumonia
  • Skin and soft tissue infections
Specimen:

Pure culture.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

PFGE will only be applied for outbreak investigations or comparative purpose. Please consult lab prior to requesting test.

Methods and Interpretation of Results:

Fingerprints are compared to a national database.

Turnaround Time:

10 calendar days if request is urgent, otherwise, 30 calendar days batched routine.

Contact:
Phone: (204) 789-5000
Fax: (204) 789-5020
References:
  1. 1. Mulvey, M.R., et al. 2001. Development of a Canadian standardized protocol for subtyping methicillin-resistant Staphylococcus aureus using pulsed-field gel electrophoresis. J Clin Microbiol 39:3481-5.
Guidelines:
Related Information: