Detection of antibodies against Machupo virus by ELISA<<Return to Laboratory
Detection of antibodies to New World Arenaviruses (NWA): Machupo virus infection by Enzyme Linked Immunosorbent Assay (ELISA).
- Bolivian hemorrhagic fever (BHF)
Collect in serum separator tubes (SST), can submit as aliquots in sterile 1.5-2.0 mL tube.
Store samples in refrigerator or frozen until being shipped for testing. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Avoid freezing whole blood samples.
Store samples in refrigerator or frozen until being shipped for testing. Ship frozen specimens on dry ice and refrigerated samples on wet ice. Shipping of samples suspected of containing Risk Group 4 pathogens shall be done by a TDG certified individual in accordance with TDG regulations. If applicable, initiate an Emergency Response Assistance Plan (ERAP) notification 24 hours prior to shipping. To initiate an ERAP notification, please click the link below to access the transport flow chart for Risk Group 4 pathogens or call 1-866-262-8433 (available 24/7) for assistance.
Symptomatic and travel to New World Arenavirus endemic areas or contact to travellers from these areas.
Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.
All patient history must be included; sample without adequate patient history to justify testing are subject to rejection. Contact Special Pathogens prior to submission of sample to ensure appropriate shipping documentation is generated.
For diagnosis of New World Arenavirus infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of antibodies to New World Arenaviruses, (ii) presence of New World Arenavirus RNA by RT-PCR, and (iii) isolation of New World Arenavirus.
2 calendar days.