Molecular Detection of Lymphocytic Choriomeningitis Virus (LCMV)

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Reference Details

Description:

PCR amplification of LCMV virus specific genome.

Test Category:
Molecular Detection
Pathogen:
Lymphocytic choriomeningitis virus (LCMV)
Illnesses and Diseases:
  • Lymphocytic Choriomeningitis (LCM)
Specimen:

Whole blood, serum, CSF, fresh frozen tissues – minimum sample 0.5 mL.  Formalin-fixed or paraffin-embedded tissues.

Collection Method:

Serum: collect in serum (red top) or serum separator tubes (SST), centrifugation of serum tubes is not needed and should be avoided.

Whole blood: collect in EDTA, heparin or citrate tubes, can submit as aliquots in sterile 1.5-2.0 mL screw cap tubes.

CSF: Collect in sterile 1.5-2.0 mL tube.

Tissues: Fresh frozen tissues should be placed in plastic containers. Tissues that are formalin-fixed should also be sent in plastic containers and clearly identified as being in formalin. Paraffin-embedded tissues can be sent as entire blocks or four to six 10 uM sections in a plastic tube or vial.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing.  Serum can be shipped refrigerated or frozen. Avoid freezing whole blood samples. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Formalin-fixed samples can be sent at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Symptomatic and/or contact with mice or their droppings.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Methods and Interpretation of Results:

For diagnosis of LCMV infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of LCMV -specific antibody titres, (ii) presence of LCMV RNA by PCR, and (iii) isolation of LCMV virus.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6019 or (204) 789-7032
Fax: (204) 789-2140
References:
Guidelines: