Isolation of Lymphocytic Choriomeningitis Virus (LCMV)

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Reference Details

Description:

Isolation of Lymphocytic Choriomeningitis Virus (LCMV)

Test Category:
Isolation
Pathogen:
Lymphocytic choriomeningitis virus (LCMV)
Illnesses and Diseases:
  • Lymphocytic Choriomeningitis (LCM)
Specimen:

Whole blood, serum, CSF.

Collection Method:

Collect whole blood in EDTA tubes, can submit as aliquots in sterile 1.5-2.0 mL tube.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing.  Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Avoid freezing whole blood samples.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Symptomatic and/or contact with mice or their droppings.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Methods and Interpretation of Results:

For diagnosis of LCMV infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of LCMV -specific antibody titers, (ii) presence of LCMV RNA by PCR, and (iii) isolation of LCMV virus.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6019 or (204) 789-7032
Fax: (204) 789-2140
References:
Guidelines: