Molecular subtyping

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*Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025).

Requisition Forms

Reference Details

Description:

Molecular subtyping of Listeria spp.

Test Category:
Molecular Differentiation
Pathogen:
Listeria spp.
Laboratory:
Illnesses and Diseases:
  • Listeriosis
Specimen:

Isolate from blood, urine, and sterile body fluid cultures.

Collection Method:

Isolate provided on culture medium or food/environmental sample.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.  Clinical specimens should be sent using proper shipping conditions for the type of specimen.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Septicemia, meningitis, gastroenteritis, neonatal infections.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

Major goals of the Listeriosis Reference Service include investigation of listeriosis cases and maintenance of a national collection of isolates. The LRS intends to create a comprehensive molecular epidemiological database of all isolates in Canada for use as a resource for outbreak investigations, research and other microbiological investigations. Molecular profiles are being established and stored for clinical, food, environmental, and possibly animal strains of L. monocytogenes. The LRS pursues research activities for investigation and implementation of other molecular methods for characterizing L. monocytogenes isolates. Next-generation typing schemes (including whole genome sequencing) have been implemented for routine typing. The LRS participates in PulseNet Canada for real-time molecular surveillance for listeriosis. The molecular epidemiological data, timely coordination and exchange of information should help to reduce the incidence of listeriosis in Canada. The LRS also participates in Listeria research projects promoting public health and is available for consultation on Listeria-focussed activities.

Methods and Interpretation of Results:

Whole Genome MLST analysis*

Whole Genome SNV analysis

Data will be forwarded to the NML Surveillance, Outbreak Detection and Response section for interpretation according to PulseNet Canada guidelines.  Please contact us using the information below for further information on interpretation guidelines.

Turnaround Time:

21 calendar days for WGS performed in-house.  5 calendar days for analysis on samples sequenced provincially.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Contact:
Phone: (204) 789-5067 or (204) 789-7605
Fax: (204) 789-5012
References: