Enzyme Linked Immunosorbent Assay (ELISA) -IgM

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025).

Requisition Forms

Reference Details

Description:

Detection of IgM antibodies to Leptospira spp. by ELISA.

Test Category:
Serology
Pathogen:
Leptospira spp.
Illnesses and Diseases:
  • Leptospirosis
Specimen:

Fresh human serum. Minimum volume of serum required is 0.25 mL. Hyperlipaemic, haemolysed or contaminated sera may yield erroneous results.

 

Collection Method:

Collect blood in serum separator tubes.

Specimen Processing, Storage and Shipping:

Transfer an aliquot of serum to a 1.5 mL screw-top vial with O-ring (Sarstedt vial). Store specimen refrigerated up to five days or store frozen until shipped for testing. Ship frozen on dry ice or with freezer packs.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Symptoms of Leptospira spp. infection may include fever, headache, chills, severe malaise, skin rash, vomiting, diarrhea, myalgia, conjunctival suffusion, jaundice, kidney and/or liver failure, meningitis and chest pains. Contact with an infected animal (dogs, cattle, raccoons, rodents, etc.) or recent travel to locations known to be endemic for Leptospira spp.

Accompanying Documentation:

Completed Requisition for Serological Testing for Selected Zoonotic Agents.

Comments:

Specimens may be subject to rejection if they are not the appropriate sample type, have insufficient volume or are not accompanied by relevant patient information or clinical history.

Initiation of antibiotic treatment prior to testing may result in decreased antibody production which will affect the outcome of serological testing.

As with any laboratory test, results of the test should be interpreted with consideration of all available laboratory and clinical findings.

Methods and Interpretation of Results:

Commercial IgM ELISA kit. This is a qualitative test for the detection of IgM antibodies to Leptospira spp. in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with leptospirosis. Samples, which are positive or equivocal by ELISA, are subject to confirmation by the Microagglutination test (MAT).

Turnaround Time:

21 calendar days (ELISA result only). Up to 30 calendar days if specimen requires further testing by MAT.

Contact:
Phone: (204) 789-7434 or (204) 789-6070
Fax: (204) 789-2082
References:
  1. Levett, P.N. Leptospirosis (2001) Clin. Micro. Rev. (14): 296-326.
Guidelines:
Related Information: