Amantadine Susceptibility Testing by Sequencing

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Susceptibility testing to Amantidine for Influenza A virus.

Test Category:
Susceptibility Testing
Pathogen:
Influenza A virus
Illnesses and Diseases:
  • Influenza
Specimen:

Viral isolate, minimum volume 0.5 mL required, if no additional testing requested.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Keep samples refrigerated until shipped for testing. Ship on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected or confirmed influenza infection.

Accompanying Documentation:

Completed requisition (current Drug Susceptibility Testing for Influenza Virus requisition form). Sender lab name, address, and telephone number must be included for samples to be processed. Also include identifier (sender lab number), patient date of birth, patient gender, type of specimen, and date collected. Under additional information, please include if possible/applicable: passage history, patient travel history, extent of influenza activity, and date of collection relative to antiviral use.

Comments:

N/A

Methods and Interpretation of Results:

Isolate susceptibility to antivirals is determined by sequence analysis. For sequence analysis, specific regions of the viral genome are amplified by PCR and sequenced; comparison of the sequence data with known wild-type sequences reveals the presence of any mutations known to cause resistance to the antiviral.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6045
Fax: (204) 789-2082
References:
  1. Hurt, AC et al. Resistance to anti-influenza drugs: adamantanes and neuraminidase inhibitors. Expert Rev. Anti Infect. Ther. (2006) 4: 795-805.