Genotyping by Luminex - Mucosal Types

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

PCR and genotyping by in-house Luminex assay on mucosal Human Papilloma Viruses.

Test Category:
Genotyping
Pathogen:
Human Papilloma virus (HPV)
Illnesses and Diseases:
  • Genital warts
Specimen:

Genital, anal or oro-pharyngeal samples. Tissue biopsies. The minimum volume required is 1/4 of the total sample for liquid based cytology (LBC) samples and the entire collected sample for cytobrushes or swabs.

Collection Method:

Cytobrush, lavage or LBC media (PreservCyt, Surepath) for genital, anal and oro-pharyngeal specimens.  Swab or biopsy for condyloma acuminatum or other visible lesions.  Fresh biopsy or fixed tissue of any lesion.

Specimen Processing, Storage and Shipping:

Place cytobrushes or swabs in 2 mL of transport medium.  Send no less than 1/4 of LBC specimen volume.  Specimens collected by cytobrush, lavage and swab may be stored and shipped refrigerated to NML within 48h of specimen collection or must be stored and shipped frozen. LBC specimens may be stored at room temperature or 4°C and shipped at room temperature.  Fresh biopsies must be stored frozen at all times and shipped on dry ice.  Fixed tissue may be stored and shipped at room temperature. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

This service is offered for reference and confirmation only. Samples for routine cervical cancer screening or ASCUS triage are not accepted unless part of pre-arranged collaborative study.

Accompanying Documentation:

Completed "Viral STI, Polyoma and Herpesvirus Testing" requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age, sex, specimen reference #, type of specimen, date collected, test requested, reason for testing (eg. Colposcopy, PAP test triage, suspected genital warts, or follow up to treatment), and any other relevant clinical information.

Comments:

N/A

Methods and Interpretation of Results:

Nested PCR followed by genotyping using Luminex technology (1) (www.luminexcorp.com). The precise HPV type(s) are reported. Presence of additional types other than the ones reported cannot be excluded. The detection limit is between 10 and 100 copies for most mucosal HPV types. This assay detects the following HPV types: High Risk: 16, 18, 26, 30, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68, 68, 69, 70, 73, 82, 85, 97 (Sufficient or limited evidence for human carcinogenesis according to IARC, Bouvard et al Lancet Oncol. 2009; 10:321-322) and other types of low or unknown risk: 6, 11, 13, 32, 40, 42, 43, 44, 54, 61, 62, 71, 72, 81, 83, 84, 86, 87, 89, 90, 91. Please note that when testing formalin-fixed specimens, the presence of HPV may not be detected due to the decreased sensitivity of the assay. Direct sequencing is also available on special request. Call in advance for information.

Turnaround Time:

21 calendar days. Please contact lab to inquire about shorter turn around time.

Contact:
Phone: (204) 789-6024
Fax: (204) 318-2222
References:
  1. Zubach V, Smart G, Ratnam S, Severini A. 2011. Novel microsphere-based method for the detection and typing of 46 mucosal human papillomavirus types. J. Clin Microbiol. 50(2): 460-4