Genotyping by Sequencing - Cutaneous Types

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Requisition Forms

Reference Details

Description:

Array of conventional PCRs and genotyping by sequencing of Human Papilloma Viruses.

Test Category:
Genotyping
Pathogen:
Human Papilloma virus (HPV)
Illnesses and Diseases:
  • Genital warts
Specimen:

Skin lesions or biopsies.

Collection Method:

Swab, cytobrush, fresh biopsies or fixed tissue.

Specimen Processing, Storage and Shipping:

Place cytobrushes or swabs in 2 mL of transport medium. Specimens collected by cytobrush or swab may be stored and shipped refrigerated to NML within 48h of sample collection or must be stored and shipped frozen. Fresh biopsies must be stored frozen at all times and shipped on dry ice. Fixed tissue may be stored and shipped at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspect human papilloma virus infection with cutaneous types.

Accompanying Documentation:

Completed "Viral STI, Polyoma and Herpesvirus Testing" requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age, sex, specimen reference #, type of specimen, date collected, test requested, reason for testing and any other relevant clinical information.

Comments:

Special request.  Not for routine testing of common skin warts.  Contact the laboratory prior to sending specimens.  Testing will be performed the last week of every month.

Methods and Interpretation of Results:

Array of PCR methods (1,2) followed by direct sequencing. The precise HPV type is reported. Presence of additional types other that the one reported cannot be excluded. Detection limit varies with the HPV type. Please, note that not all known cutaneous types can be detected by the PCR methods available. Also note that when testing formalin-fixed specimens, the presence of HPV may not be detected due to the decreased sensitivity of the assay.

Turnaround Time:

Test is done by special request; inquire with lab for turnaround times.

Contact:
Phone: (204) 789-6024
Fax: (204) 318-2222
References:
  1. Brink et al. Development of a General Primer PCR Reverse Line Blotting System for Detecton of Beta and Gamma Cutaneous Human Papillomavirus. 2005. J. Clin. Microbiol. 43:5581-5587
  2. Berkhout, R.J.M. et al. Nested PCR Approach for Detection and Typing of Epidermodysplasia Verriformis-Associated Human Papillomavirus Types in Cutaneous Cancers from Renal Transplant Recipients. 1995. J. Clin. Microbiol. 33:360-695.