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Molecular Detection by PCR

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Requisition Forms

Reference Details


Detection of human herpesvirus-8 (HHV-8) by conventional PCR.

Test Category:
Molecular Detection
Human Herpesvirus-8 (HHV-8)
Illnesses and Diseases:
  • Kaposi's sarcoma

Whole blood, serum or plasma.  Minimum volume required for either whole blood, serum or plasma is 1.0 mL.

Collection Method:

Submit whole blood, serum or plasma in a sterile microcentrifuge tube.

Specimen Processing, Storage and Shipping:

Store whole blood samples refrigerated until shipped for testing.  Ship whole blood on wet ice. Store plasma or serum samples frozen until shipped for testing.  Ship plasma or serum frozen on dry ice. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected Human Herpesvirus-8 infection.

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition, including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, test(s) requested. Indicate the type of specimen and date collected.



Methods and Interpretation of Results:

Detection of HHV-8 DNA using nested PCR.

Turnaround Time:

21 calendar days.

Phone #: (204) 789-6512
Fax: (204) 318-2222

Spira TJ, Lam L, Dollard SC, et al. (2000) Comparison of serologic assays and PCR for diagnosis of human herpesvirus 8 infection. J Clin Microbiol 38(6):2174-80.

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