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Molecular Detection by RT-PCR

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Requisition Forms

Reference Details


Molecular detection of Hepatitis E virus by Real Time and conventional RT-PCR.

Test Category:
Molecular Detection
Hepatitis E virus (HEV)
Illnesses and Diseases:
  • Hepatitis E

Serum, plasma or fecal sample.  Minimum volume required for serum or plasma – 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected Hepatitis E virus infection.   Serologically confirmed acute or chronic Hepatitis E virus infection. If sample is anti-HEV IgM negative/IgG positive, please provide justification on the requisition for molecular testing (i.e., immunosuppression, transplant, etc.).

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.



Methods and Interpretation of Results:

Conventional RT PCR with primer sets amplifying all HEV genotypes at the ORF1 region.

Turnaround Time:

19 calendar days.

Phone #: (204) 789-6061
Fax: (204) 318-2222
  1. Drexler JF, Seelen A, Corman VM, Tateno AF, Cottontail V, Zerbinati RM, Gloza-Rausch F, Klose SM, Adu-Sarkodie Y, Oppong SK, Kalko EKV, Osterman A, Rasche A, Adam A, Müller MA, Ulrich RG, Leroy EM, Lukashevand AN, Drosten C. Bats Worldwide Carry Hepatitis E Virus-Related Viruses That Form a Putative Novel Genus within the Family Hepeviridae. J Virol. 2012 Sep; 86(17): 9134–9147.
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