Language Selection


Genotyping by RT-PCR

<<Return to Laboratory

Requisition Forms

Reference Details


Genotyping of Hepatitis E virus by conventional RT-PCR followed by sequencing.

Test Category:
Hepatitis E virus (HEV)
Illnesses and Diseases:
  • Hepatitis E

Serum, plasma, or fecal sample. Minimum volume required for serum or plasma is 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected Hepatitis E virus infection.  Serologically confirmed acute or chronic Hepatitis E virus infection. If sample is anti-HEV IgM negative/IgG positive, please provide justification on the requisition for molecular testing (i.e., immunosuppression, transplant, etc.)”.

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.



Methods and Interpretation of Results:

Conventional RT PCR with primer sets amplifying all HEV genotypes at the ORF1 region.

Turnaround Time:

19 calendar days.

Phone #: (204) 789-6512
Fax: (204) 318-2222
  1. Drexler JF, Seelen A, Corman VM, Tateno AF, Cottontail V, Zerbinati RM, Gloza-Rausch F, Klose SM, Adu-Sarkodie Y, Oppong SK, Kalko EKV, Osterman A, Rasche A, Adam A, Müller MA, Ulrich RG, Leroy EM, Lukashevand AN, Drosten C. Bats Worldwide Carry Hepatitis E Virus-Related Viruses That Form a Putative Novel Genus within the Family Hepeviridae. J Virol. 2012 Sep; 86(17): 9134–9147.
  2. Baylis SA, Adlhoch C, Child L, and the HEV Sequencing Study Group. An Evaluation of Hepatitis E Virus Molecular Typing Methods. Clin Chem 2021 Dec; 68(1):181-191.
Related Information: