Genotyping by RT-PCR
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Reference Details
Genotyping of Hepatitis E virus by conventional RT-PCR followed by sequencing.
- Hepatitis E
Serum, plasma, or fecal sample. Minimum volume required for serum or plasma is 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis E virus infection. Serologically confirmed acute or chronic Hepatitis E virus infection. If sample is anti-HEV IgM negative/IgG positive, please provide justification on the requisition for molecular testing (i.e., immunosuppression, transplant, etc.)”.
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
N/A
Conventional RT PCR with primer sets amplifying all HEV genotypes at the ORF1 region.
19 calendar days.
- Drexler JF, Seelen A, Corman VM, Tateno AF, Cottontail V, Zerbinati RM, Gloza-Rausch F, Klose SM, Adu-Sarkodie Y, Oppong SK, Kalko EKV, Osterman A, Rasche A, Adam A, Müller MA, Ulrich RG, Leroy EM, Lukashevand AN, Drosten C. Bats Worldwide Carry Hepatitis E Virus-Related Viruses That Form a Putative Novel Genus within the Family Hepeviridae. J Virol. 2012 Sep; 86(17): 9134–9147.
- Baylis SA, Adlhoch C, Child L, and the HEV Sequencing Study Group. An Evaluation of Hepatitis E Virus Molecular Typing Methods. Clin Chem 2021 Dec; 68(1):181-191.