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Anti-HDV Enzyme Immunoassay (EIA)

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details


Detection of IgG antibodies to the Hepatitis Delta virus by enzyme immunoassay (EIA).

Test Category:
Hepatitis Delta virus (HDV)
Illnesses and Diseases:
  • Hepatitis D

Serum or plasma sample. Minimum volume required for serum or plasma – 0.5 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected Hepatitis Delta virus infection.

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.


Anti-HDV positive specimens are automatically reflexed to HDV RNA testing, dependent on sample volume. Anti-HDV negative specimens are not automatically tested for HDV RNA. RNA testing may be requested with justification (immunosuppression, etc.).

Methods and Interpretation of Results:

Microplate EIA, commercially available assay, research use only (RUO; Beijing Wantai).  A positive anti-HDV IgG result may indicate acute, chronic, or resolved HDV infection.

Turnaround Time:

15 calendar days. Borderline samples that need to be repeated have an extended turnaround time of 21 calendar days.

Phone #: (204) 789-6512
Fax: (204) 318-2222
  1. M. Fiedler, M. Roggendorf. Immunology of HDV infection. Current Topics in Microbiology and Immunology. 2006; 307:187-209.
  2. A. Olivero, A. Smedile. Hepatitis delta virus diagnosis. Seminars in Liver Diseases. 2012; 32:220-227.
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