Anti-HDV Enzyme Immunoassay (EIA)
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Requisition Forms
Reference Details
Detection of IgG antibodies to the Hepatitis Delta virus by enzyme immunoassay (EIA).
- Hepatitis D
Serum or plasma sample. Minimum volume required for serum or plasma – 0.5 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Presence of Hepatitis B Virus and Suspected Hepatitis Delta virus infection.
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
Anti-HDV positive specimens are automatically reflexed to HDV RNA testing, dependent on sample volume. Anti-HDV negative specimens are not automatically tested for HDV RNA. RNA testing may be requested with justification (immunosuppression, etc.).
Microplate EIA, commercially available assay, research use only (RUO; Beijing Wantai). A positive anti-HDV IgG result may indicate acute, chronic, or resolved HDV infection.
15 calendar days. Borderline samples that need to be repeated have an extended turnaround time of 21 calendar days.
- M. Fiedler, M. Roggendorf. Immunology of HDV infection. Current Topics in Microbiology and Immunology. 2006; 307:187-209.
- A. Olivero, A. Smedile. Hepatitis delta virus diagnosis. Seminars in Liver Diseases. 2012; 32:220-227.