Viral Load Quantification

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Requisition Forms

Reference Details

Description:

Determination of Hepatitis Delta viral load by quantitative RT-PCR.

Test Category:
Molecular Quantification
Pathogen:
Hepatitis Delta virus (HDV)
Illnesses and Diseases:
  • Hepatitis D
Specimen:

Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected Hepatitis Delta virus infection. Serology results must show markers to the Hepatitis Delta virus. HDV qualitative RT-PCR must be positive.

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Comments:

N/A

Methods and Interpretation of Results:

In-house real-time RT-PCR for the follow-up of patients with chronic HDV infection.

Turnaround Time:

19 calendar days.

Contact:
Phone: (204) 789-6061; HCV (204) 789-5063
Fax: (204) 789-2082
References:
  1. Le Gal F, Gordien E, Affolabi D, et al., Quantification of hepatitis delta virus RNA in serum by consensus real-time PCR indicates different patterns of virological response to interferon therapy in chronically infected patients. J Clin Virol 2005; 43(5):2363-2369.
  2. Shang D, Hughes SA, Horner M, et al., Development and validation of an efficient in-house real-time reverse transcription polymerase chain reaction assay for the quantitative detection of serum hepatitis delta virus RNA in a diverse South London population. J Virol Methods 2012;184:55-62.
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