Molecular Detection by RT-PCR
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Reference Details
Molecular detection of Hepatitis Delta virus by conventional RT-PCR.
- Hepatitis D
Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis Delta virus infection. Serology results must show markers to the Hepatitis D virus.
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
Only samples that are positive for anti-HDV antibodies will be tested. Samples submitted for anti-HDV serology testing are automatically reflexed to HDV RNA testing, if positive for antibodies. Anti-HDV negative specimens are not automatically tested for HDV RNA. RNA testing may be requested with justification (immunosuppression, etc.).
Conventional RT-PCR of the small HDV antigen-coding region.
19 calendar days.
- Theamboonlers A, Hansurabhanon T, Verachai V, Chongsrisawat V, Poovorawan Y. Hepatitis D virus infection in Thailand: HDV genotyping by RT-PCR, RFLP and direct sequencing. Infection. 2002;30:140-4.