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Genotyping by Sequence Analysis

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Requisition Forms

Reference Details


Genotyping of Hepatitis Delta virus by conventional RT-PCR followed by DNA sequencing.

Test Category:
Hepatitis Delta virus (HDV)
Illnesses and Diseases:
  • Hepatitis D

Serum or plasma sample. Minimum volume required for serum or plasma is 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected Hepatitis Delta virus infection. Serology results must show markers to the Hepatitis Delta virus (anti-HDV IgG).

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.


Repeated testing of HDV genotype for chronic hepatitis D patients is not warranted unless superinfection/re-infection is suspected.

Methods and Interpretation of Results:

Conventional RT-PCR of the small HDV antigen coding region followed by sequencing and phylogenetic analysis in comparison to HDV genotype reference sequences.

Turnaround Time:

19 calendar days.

Phone #: (204) 789-6512
Fax: (204) 318-2222
  1. Dény P. Hepatitis delta virus genetic variability: from genotypes I, II, III to eight major clades? Curr Top Microbiol Immunol. 2006;307:151-71.
  2. Theamboonlers A, Hansurabhanon T, Verachai V, Chongsrisawat V, Poovorawan Y. Hepatitis D virus infection in Thailand: HDV genotyping by RT-PCR, RFLP and direct sequencing. Infection. 2002 Jun;30(3):140-4.
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