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Anti-HBs Enzyme Immunoassay (EIA)

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Requisition Forms

Reference Details


Detection of antibodies to the Hepatitis B surface antigen by enzyme immunoassay (EIA).

Test Category:
Hepatitis B virus (HBV)
Illnesses and Diseases:
  • Hepatitis B

Serum or plasma sample. Minimum volume required for serum or plasma – 0.5 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Vaccination against HBV or suspected past Hepatitis B infection.

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.



Methods and Interpretation of Results:

Automated EIA assay (Roche Elecsys). This is a quantitative test for vaccine-induced or naturally acquired immunity to HBV infection. It is widely accepted that at least 10 mIU/mL is protective against HBV infection. An enduring response to immunization usually is > 100 mIU/mL.

Turnaround Time:

15 calendar days.

Phone #: (204) 789-6512
Fax: (204) 318-2222
  1. Huzly D, Schenk T, Jilg W, Neumann-Haefelin D. Comparison of nine commercially available assays for quantification of antibody response to hepatitis B virus surface antigen. J Clin Microbiol. 2008 Apr;46(4):1298-306.